Mainly used for blood analysis (counting leucocytes, electrolyte, and thrombocytes) & to count cells, bacteria, fungus, spores or other particles in suspension under microscope, Made of special optical glass according to DIN 12847 standard. The slide surface is divided into 2 large fields( for inscriptions) & 3 narrow ridges (ground & polished). Double ruling & all group squares with triple boundary lines for counting, Formula : Particles per ul volume - Counted particles (counted surface x chamber depth x Dilution)