Selective medium used for isolating pathogenic staphylococci from clinical specimens, food and other materials
of sanitary importance. This medium is intended as an aid in the diagnosis, requiring further identification tests
to complete the diagnostic results.
Mannitol Salt Agar (MSA) is a medium for the selective isolation of staphylococci while allowing differentiation
of mannitol fermenting from non fermenting staphylococci.
This medium is prepared according to recommendations of the harmonized USP/EP/JP method for the detection
of S. aureus in non sterile pharmaceutical products.
TYPICAL FORMULA :
Pancreatic Digest of Casein : 5.0 g/litre
Peptic Digest of Animal Tissue : 5.0 g/litre
Beef Extract : 1.0 g/litre
D-Mannitol : 10.0 g/litre
Sodium Chloride : 75.0 g/litre
Phenol Red : 0.025 g/litre
Agar : 15.0 g/litre
Final pH : 7.4 ± 0.2 at 25°C
